Coding

Part:BBa_K3841015:Design

Designed by: Emil Ørsted Christensen   Group: iGEM21_DTU-Denmark   (2021-09-27)


Particulate methane monooxygenase alpha subunit (PmoB) fused to Venus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 619
    Illegal XbaI site found at 1153
    Illegal PstI site found at 1474
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1474
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 334
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 619
    Illegal XbaI site found at 1153
    Illegal PstI site found at 1474
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 619
    Illegal XbaI site found at 1153
    Illegal PstI site found at 1474
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used GeneWiz to codon optimize the sequence for expression in Komagataella phaffii. Additionally, we removed any BsaI and BsmBI sites within the coding sequence.


Source

Codon optimized pMMO from https://www.uniprot.org/uniprot/G1UBD1 & Codon optimized Venus from https://parts.igem.org/Part:BBa_K1907000

References